Doxycycline-hydrochloride _ MMP 抑制剂 - MedChemExpress_图文...

来自 : 发布时间：2024-11-27

•Agonists•ScreeningLibrarieswww.MedChemExpress.cnDoxycyclinehydrochlorideCat.No.:HY-N0565ACASNo.:10592-13-9分⼦式:C₂₂H₂₅ClN₂O₈分⼦量:480.9作⽤靶点:MMP作⽤通路:MetabolicEnzyme/Protease储存⽅式:PleasestoretheproductundertherecommendedconditionsintheCOA.BIOLOGICALACTIVITY⽣物活性Doxycyclinehydrochloride是合成的四环素类衍⽣物，具有抗菌活性。IC50 TargetMMP体外研究Doxycyclineshowsexcellenteffectivenessandtime-dependentcharacteristicsagainstM.gallisepticumstrainS6invitro[2].Osteoblastsexposedtothecompositecontaining25μg/mLdoxycycline(DOX)/β-cyclodextrin(βCD)hasincreasedcellproliferation(p 0.05)comparedtocontrolosteoblastculturesatallexperimentaltimepoints,reachingamaximuminthesecondweek.Alkalinephosphatase(AP)activityandcollagensecretionlevelsarealsoelevatedinosteoblastsexposedtotheDOX/βCDcomposite(p 0.05vs.controls)andreachamaximumafter14days[3].Doxycycline(20nM)inhibitsECM(extracellularmatrix)productionandremodelinginbothSMC(smoothmusclecell)typesofcultures,andsynthesisofcollagensandisoprenylatedproteinsinSMC-Ch(acholesterol-richdiet)isahigherthaninSMC-C(astandarddiet)[4].体内研究Inheterozygous(HT)Col3a1knockoutmice,after3monthsoftreatmentwithdoxycyclineorplacebo,9-month-oldHTorwild-type(WT)micearesubjectedtosurgicalstressingoftheaorta.A3-foldincreaseinstress-inducedaorticlesionsfoundinuntreatedHTmice1weekafterintervention(cumulativescore4.5±0.87versus1.3±0.34inWT,p[1].PROTOCOLGelatin(0.1%(w/v)isaddedtostandardLaemmLiacrylamidepolymerizationmixture.Tissueextractismixed1:2withsamplebuffer[250mMTris-ClpH6.8,10%(w/v)SDS,20%(v/v)glycerol,0.005%(w/v)KinaseAssay[1]1/3MasterofSmallMolecules—您⾝边的抑制剂⼤师