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当前位置： 首页 > 产品中心 > Small_molecule > Medchemexpress/D-荧光素（同义词：D-（-）-荧光素；萤火虫荧光素）/HY-12591A/50mg
商品详细Medchemexpress/D-荧光素（同义词：D-（-）-荧光素；萤火虫荧光素）/HY-12591A/50mg
Medchemexpress/D-荧光素（同义词：D-（-）-荧光素；萤火虫荧光素）/HY-12591A/50mg
Medchemexpress/D-荧光素（同义词：D-（-）-荧光素；萤火虫荧光素）/HY-12591A/50mg
商品编号： HY-12591A-10mM*1mLinDMSO
品牌： MedChemExp
市场价： ¥1100.00
美元价： 660.00
产地： 美国(厂家直采)
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产品分类： 小分子
公司分类： Small_molecule
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商品介绍
D-luciferinisthenaturalsubstrateofluciferasesthatcatalyzetheproductionoflightinbioluminescentinsects.
Description

D-luciferinisthenaturalsubstrateofluciferasesthatcatalyzetheproductionoflightinbioluminescentinsects.

InVitro

D-luciferinisthenaturalsubstrateoftheenzymeluciferase(Luc),thatcatalyzestheproductionofthetypicalyellowgreenlightoffireflies.ThepresentreviewcoversthesynthesisofD-luciferinandderivativesoranaloguesthataresubstratesorinhibitorsoftheluciferasefromtheAmericanfireflyPhotinuspyralis,theenzymemorefrequentlyusedintechniquesofinvitroandopticalimaging[1].

InVivo

Bioluminescenceimaging(BLI)usingthefireflyluciferase(Fluc)asareportergeneandD-luciferinasasubstrateiscurrentlythemostwidelyemployedtechnique.ThetotalsignalintensityisplottedagainstthetimeafterD-luciferininjectiontogenerateatime-intensitycurve.Inadditiontothepeaksignal,thesignalsatfixedtimepoints(5,10,15,and20 min)afterD-luciferininjectionaredeterminedasalternativestothepeaksignal.Thesignalinagiventime-intensitycurveisnormalizedforthepeaksignalinthecurvetorepresentthepatternoftemporalchangesafterD-luciferininjection[2].

References

  • [1].GiuseppeMeroni,etal.D-Luciferin,derivativesandanalogues:synthesisandinvitro/invivoluciferase-catalyzedbioluminescentactivity.ARKIVOC2009(i)265-288.

    [2].InoueY,etal.Timingofimagingafterd-luciferininjectionaffectsthelongitudinalassessmentoftumorgrowthusinginvivobioluminescenceimaging.IntJBiomedImaging.2010;2010:471408.

PreparingStockSolutions
ConcentrationVolumeMass1mg5mg10mg
1mM3.5674mL17.8368mL35.6735mL
5mM0.7135mL3.5674mL7.1347mL
10mM0.3567mL1.7837mL3.5674mL
Pleaserefertothesolubilityinformationtoselecttheappropriatesolvent.
AnimalAdmiNISTration
[2]

D-luciferinispreparedinphosphate-bufferedsaline(Mice)[2].

Mice[2]
InvivoBLIisperformedusingacooledcharge-coupleddevicecamerasystem(IVISImagingSystem100)3,5,7,10,12,14,19,21,24,and28daysaftertheinoculationofHCT116-Luccells.Miceareinjectedwith75 mg/kgD-luciferinin100 Lofphosphate-bufferedsalinesubcutaneouslynearthescapulaandwereplacedinthelight-tightchamberoftheimagingsystemunderisofluraneanesthesia.Beginning5 minafterinjection,dorsalluminescentimageswithanexposuretimeof1 sareacquiredsequentiallyatarateofoneimageperminuntil20 minafterD-luciferininjection.Dataacquisitioniscontinueduntil40 minpostinjectionondays3or5anduntil25 minonday7,becauseoftheprolongedtimecourseoflightemission.Binningis4andthefieldofviewis15 cm.
MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.

References

  • [1].GiuseppeMeroni,etal.D-Luciferin,derivativesandanalogues:synthesisandinvitro/invivoluciferase-catalyzedbioluminescentactivity.ARKIVOC2009(i)265-288.

    [2].InoueY,etal.Timingofimagingafterd-luciferininjectionaffectsthelongitudinalassessmentoftumorgrowthusinginvivobioluminescenceimaging.IntJBiomedImaging.2010;2010:471408.

MolecularWeight

280.32

Formula

C₁₁H₈N₂O₃S₂

CASNo.

2591-17-5

Storage
Powder-20°C3years
 4°C2years
Insolvent-80°C6months
 -20°C1month
Shipping

RoomtemperatureincontinentalUS;mayvaryelsewhere

Solvent&Solubility

DMSO:14.7mg/mL

*"<1 mg/ml"="" means="" slightly="" soluble="" or="" insoluble.="" "≥"="" means="" soluble,="" but="" saturation="">

Purity:98.11%