Customer Validation
- •Obesity (Silver Spring). 2017 Sep;25(9):1532-1539.
- •Am J Transl Res. 2016 Sep 15;8(9):3955-3962.
- •Am J Transl Res. 2016 Jun 15;8(6):2562-74.
| Description |
5-BrdU is a nucleoside analog, and competes with thymidine for incorporation into DNA, and is used in the detection of proliferating cells. |
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| In Vitro |
Bromodeoxyuridine induces a progressive, dose-responsive suppression of cancer cell line and cancer stem cell population expansion RG2 rat glioma cells. In H9 cells and BJ fibroblasts, bromodeoxyuridine alters the cell cycle profile[1]. BrdU is stably integrated into the DNA, and thus can be used in assessment of cell proliferation and other cell procession[2]. |
| In Vivo |
Bromodeoxyuridine (300 mg/kg, i.p. or 0.8 mg/mL, p.o.) significantly slows tumor progression in rat glioma RG2 tumor model[1]. |
| Clinical Trial |
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| References |
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| Preparing Stock Solutions |
Please refer to the solubility information to select the appropriate solvent.
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| Cell Assay
[1] |
Cultures are initially plated at 2000 cells/cm2 and are quantified with a Z2 Coulter Counter. RG2 rat glioma cells are treated once with 0, 1, 10, or 50 µM BrdU for 24 hours, and cumulative growth curves are obtained over 18 days. Control and treated cells are quantified and replated at equal densities on days 5, 12, and 18 after treatment. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
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| References |
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| Molecular Weight |
307.1 |
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| Formula |
C₉H₁₁BrN₂O₅ |
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| CAS No. |
59-14-3 |
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| Storage |
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| Shipping | Room temperature in continental US; may vary elsewhere |
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| Solvent & Solubility |
DMSO: ≥ 41 mg/mL
* "<1 mg/ml"="" means="" slightly="" soluble="" or="" insoluble.="" "≥"="" means="" soluble,="" but="" saturation=""> |
Purity: 98.93%
COA (95 KB) HNMR (658 KB) LCMS (259 KB)
Handling Instructions (1252 KB)-
[1]. Levkoff LH, et al. Bromodeoxyuridine inhibits cancer cell proliferation in vitro and in vivo. Neoplasia. 2008 Aug;10(8):804-16.
[2]. Rothaeusler K, et al. Assessment of cell proliferation by 5-bromodeoxyuridine (BrdU) labeling for multicolor flow cytometry. Curr Protoc Cytom. 2007 Apr;Chapter 7:Unit7.31.
