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当前位置： 首页 > 产品中心 > Small_molecule > Medchemexpress/Genipin（同义词：（+）-Genipin）/HY-17389/10mM*1mL二甲基亚砜
商品详细Medchemexpress/Genipin（同义词：（+）-Genipin）/HY-17389/10mM*1mL二甲基亚砜
Medchemexpress/Genipin（同义词：（+）-Genipin）/HY-17389/10mM*1mL二甲基亚砜
Medchemexpress/Genipin（同义词：（+）-Genipin）/HY-17389/10mM*1mL二甲基亚砜
商品编号： HY-17389-10mM*1mLinDMSO
品牌： MedChemExp
市场价： ¥2480.00
美元价： 1488.00
产地： 美国(厂家直采)
公司：
产品分类： 小分子
公司分类： Small_molecule
联系Q Q： 3392242852
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商品介绍
Genipinisacellpermeableinhibitorofuncouplingprotein2(UCP2).
Description

Genipinisacellpermeableinhibitorofuncouplingprotein2(UCP2).

InVitro

Genipinstimulatsglucoseuptakeinatime-anddose-dependentmanner.Themaximaleffectisachievedat2 hwithaconcentrationof10 μM.Inmyotubes,genipinpromotesglucosetransporter4(GLUT4)translocationtothecellsurface,whichincreasesthephosphorylationofinsulinreceptorsubstrate-1(IRS-1),AKT,andGSK3β.Meanwhile,genipinincreasesATPlevels,closedKATPchannels,andthenincreasestheconcentrationofcalciuminthecytoplasminC2C12myotubes.Genipin-stimulatedglucoseuptakecouldbeblockedbyboththePI3-KinhibitorwortmanninandcalciumchelatorEGTA.Moreover,genipinincreasesthelevelofreactiveoxygenspeciesandATPinC2C12myotubes[1].Genipinincreasesmitochondrialmembranepotential,whichthenincreasesATPlevelsandclosesKATPchannels,therebystimulatinginsulinsecretioninpancreaticβ-cells.Genipinactivatesglucose-excitedPOMCneurons[2].Cytochromeccontentincreasessignificantlyinthecytosolofgenipin-treatedFaOcells.Activationofcaspase-3andcaspase-7isultimatelyresponsIBLeforgenipin-inducedapoptoticprocessinhepatomacells.ROSlevelnotablyincreasesinHep3Bcellstreatedwith200μMgenipin[3].

References

  • [1].MaC,etal.GenipinstimulatesglucosetransportinC2C12myotubesviaIRS-1andcalcium-dependentmechanism.JEndocrinol.2012Dec20.

    [2].PartonLE,etal.GlucosesensingbyPOMCneuronsregulatesglucosehomeostasisandisimpairedinobesity.Nature.2007Sep13;449(7159):228-32.

    [3].KimBC,etal.Genipin-inducedapoptosisinhepatomacellsismediatedbyreactiveoxygenspecies/c-JunNH2-terminalkinase-dependentactivationofmitochondrialpathway.BiochemPharmacol.2005Nov1;70(9):1398-407.

PreparingStockSolutions
ConcentrationVolumeMass1mg5mg10mg
1mM4.4203mL22.1014mL44.2028mL
5mM0.8841mL4.4203mL8.8406mL
10mM0.4420mL2.2101mL4.4203mL
Pleaserefertothesolubilityinformationtoselecttheappropriatesolvent.
KinaseAssay
[3]

Briefly,thepeptidesubstrateN-acetyl-Asp-Glu-Val-Asp-ρ-nitroanilide(Ac-DEVD-ρNA)isaddedtothecelllysatesinassaybuffer(50mMHEPES,pH7.4,100mMNaCl,0.1%CHAPS,10mMdithiothreitol,1mMEDTA,10%glycerol)andincubatedat37°C.Thecleavageofthesubstrateismonitoredat405nm.MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.

References

  • [1].MaC,etal.GenipinstimulatesglucosetransportinC2C12myotubesviaIRS-1andcalcium-dependentmechanism.JEndocrinol.2012Dec20.

    [2].PartonLE,etal.GlucosesensingbyPOMCneuronsregulatesglucosehomeostasisandisimpairedinobesity.Nature.2007Sep13;449(7159):228-32.

    [3].KimBC,etal.Genipin-inducedapoptosisinhepatomacellsismediatedbyreactiveoxygenspecies/c-JunNH2-terminalkinase-dependentactivationofmitochondrialpathway.BiochemPharmacol.2005Nov1;70(9):1398-407.

MolecularWeight

226.23

Formula

C₁₁H₁₄O₅

CASNo.

6902-77-8

Storage

4°C,protectfromlight

Shipping

RoomtemperatureincontinentalUS;mayvaryelsewhere

Solvent&Solubility

DMSO:≥210mg/mL

*"<1 mg/ml"="" means="" slightly="" soluble="" or="" insoluble.="" "≥"="" means="" soluble,="" but="" saturation="">

Purity:99.42%