CustomerValidation
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Description | ST2825isaMyD88homodimerizationinhibitor. |
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IC50&Target | MyD88[1] |
InVitro | ST2825blocksIL-1R/TLRsignalingbyinterferingwithMyD88homodimerization.ST2825inhibitsthisinteractioninaconcentration-dependentmannerwith~40%inhibitionofdimerizationat5μMST2825and80%inhibitionat10μMST2825[1]. |
InVivo | ST2825dose-dependentlyinhibitsIL-1β-inducedproductionofIL-6intreatedmiceafteroraladmiNISTration.TheanimalsareadministeredorallywiththeappropriatevehiclesorST2825atdosesrangingfrom50to200mg/kg,5minpriortoi.p.injectionwith20μg/kgIL-1β.ST2825exertesasignificantinhibitionofIL-1β-stimulatedproductionofIL-6at100and200mg/kg[1]. |
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PreparingStockSolutions |
Pleaserefertothesolubilityinformationtoselecttheappropriatesolvent. | ||||||||||||||||
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CellAssay [1] | ST2825isdissolvedinDMSOandstored,andthendilutedwithappropriatemedia(DMSO0.1%)beforeuse[1]. HeLacellsareseededat105cells/mLina96-welltissue-cultureplate.Afterincubatingovernight,themediumisdiscarded,andthecellsareaddedwithtissueculturemedium,10%FBS,containingST2825atconcentrationsrangingfrom0.1to10μMandDMSOat0.1%finalconcentration.Thecellsareincubatedfor6and18handthenaddedwiththeyellowXTT(0.3mg/mL)forfurther2hofincubation.Attheendoftheincubationperiods,reactionsarequantifiedbyusingaSirioSSeacmicroplatereader[1].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly. | ||||||||||||||||
AnimalAdministration [1] | ST2825isadministeredorallyas0.5%sUSPensionincarboxymethylcellulose(CMC)[1]. Mice[1] | ||||||||||||||||
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MolecularWeight | 591.51 | ||||||||||||
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Formula | C₂₇H₂₈Cl₂N₄O₅S | ||||||||||||
CASNo. | 894787-30-5 | ||||||||||||
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Shipping | RoomtemperatureincontinentalUS;mayvaryelsewhere | ||||||||||||
Solvent&Solubility | 10mMinDMSO ST2825isdissolvedinDMSO.ST2825solution(0.5μL)isadministeredviaintracerebroventricular(ICV)injectionat15minpost-injury,usinga1μLHamiltonmicrosyringe[4]. *"<1 mg/ml"="" means="" slightly="" soluble="" or="" insoluble.="" "≥"="" means="" soluble,="" but="" saturation="">1> | ||||||||||||
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Purity:99.51%