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当前位置： 首页 > 产品中心 > Small_molecule > Medchemexpress/Puromycin Dihydrochloride(Synonyms: CL13900 dihydrochloride)/HY-B1743A/50mg
商品详细Medchemexpress/Puromycin Dihydrochloride(Synonyms: CL13900 dihydrochloride)/HY-B1743A/50mg
Medchemexpress/Puromycin Dihydrochloride(Synonyms: CL13900 dihydrochloride)/HY-B1743A/50mg
Medchemexpress/Puromycin Dihydrochloride(Synonyms: CL13900 dihydrochloride)/HY-B1743A/50mg
商品编号： HY-B1743A-10mM*1mLinDMSO
品牌： MedChemExp
市场价： ¥1100.00
美元价： 660.00
产地： 美国(厂家直采)
公司：
产品分类： 小分子
公司分类： Small_molecule
联系Q Q： 3392242852
电话号码： 4000-520-616
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商品介绍
Puromycindihydrochlorideisthedihydrochloridesaltofpuromycin.PuromycinisanaminoglycosideantibioticisolatedfromthebacteriumStreptomycesalboniger.
Description

Puromycindihydrochlorideisthedihydrochloridesaltofpuromycin.PuromycinisanaminoglycosideantibioticisolatedfromthebacteriumStreptomycesalboniger.

InVitro

Puromycinblocksproteinsynthesisafteraminoacyl-sRNAformation,andatthesametimeitleadstotheaccumulationofsmallpeptides.Bothoftheseeffectsappeartobeduetothesplittingofribosome-boundpeptidyl-sRNA,4whichresultsinreleaseofincompletepeptidechains.[1].Puromycin,ananalogofthe3"endofaminoacyl-tRNA,causesprematureterminationoftranslationbybeinglinkednon-specificallytogrowingpolypeptidechains.Puromycinhastwomodesofinhibitoryaction.Thefirstisbyactingasanacceptorsubstratewhichattackspeptidyl-tRNAinthePsitetoformanascentpeptide.Thesecondisbycompetingwithaminoacyl-tRNAforbindingtotheA"site[2].Whenusedinminimalamounts,puromycinincorporationinneosynthesizedproteinsreflectsdirectlytherateofmRNAtranslationinvitro.PuromycinimmunodetectionisanadvantageousalternativetorADIoactiveaminoacidlabeling.Itallowsthedirectevaluationoftranslationactivityinsinglecellsbyimmunofluorescencemicroscopyandinheterogenouspopulationsofcellsbyfluorescenceactivatedcellsorting[3].

References

  • [1].NathansD,etal.Puromycininhibitionofproteinsynthesis:incorporationofpuromycinintopeptidechains.ProcNatlAcadSciUSA.1964Apr;51:585-92.

    [2].Miyamoto-SatoE,etal.Specificbondingofpuromycintofull-lengthproteinattheC-terminus.NucleicAcidsRes.2000Mar1;28(5):1176-82.

    [3].SchmidtEK,etal.SUnSET,anonradioactivemethodtomonitorproteinsynthesis.NatMethods.2009Apr;6(4):275-7.

PreparingStockSolutions
ConcentrationVolumeMass1mg5mg10mg
1mM1.8368mL9.1839mL18.3678mL
5mM0.3674mL1.8368mL3.6736mL
10mM0.1837mL0.9184mL1.8368mL
Pleaserefertothesolubilityinformationtoselecttheappropriatesolvent.
References

  • [1].NathansD,etal.Puromycininhibitionofproteinsynthesis:incorporationofpuromycinintopeptidechains.ProcNatlAcadSciUSA.1964Apr;51:585-92.

    [2].Miyamoto-SatoE,etal.Specificbondingofpuromycintofull-lengthproteinattheC-terminus.NucleicAcidsRes.2000Mar1;28(5):1176-82.

    [3].SchmidtEK,etal.SUnSET,anonradioactivemethodtomonitorproteinsynthesis.NatMethods.2009Apr;6(4):275-7.

MolecularWeight

544.43

Formula

C₂₂H₃₁Cl₂N₇O₅

CASNo.

58-58-2

Storage
Powder-20°C3years
 4°C2years
Insolvent-80°C6months
 -20°C1month
Shipping

RoomtemperatureincontinentalUS;mayvaryelsewhere

Solvent&Solubility

DMSO:10mM

*"<1 mg/ml"="" means="" slightly="" soluble="" or="" insoluble.="" "≥"="" means="" soluble,="" but="" saturation="">

Purity:99.63%