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当前位置： 首页 > 产品中心 > Small_molecule > Medchemexpress/大豆苷元（同义词：异黄酮）/HY-N0019/10mM*1mL二甲基亚砜
商品详细Medchemexpress/大豆苷元（同义词：异黄酮）/HY-N0019/10mM*1mL二甲基亚砜
Medchemexpress/大豆苷元（同义词：异黄酮）/HY-N0019/10mM*1mL二甲基亚砜
Medchemexpress/大豆苷元（同义词：异黄酮）/HY-N0019/10mM*1mL二甲基亚砜
商品编号： HY-N0019-10mM*1mLinDMSO
品牌： MedChemExp
市场价： ¥1100.00
美元价： 660.00
产地： 美国(厂家直采)
公司：
产品分类： 小分子
公司分类： Small_molecule
联系Q Q： 3392242852
电话号码： 4000-520-616
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商品介绍
Daidzeinisasoyisoflavone,whichactsasaPPARactivator.
Description

Daidzeinisasoyisoflavone,whichactsasaPPARactivator.

IC50&Target

PPAR[1]

InVitro

In3T3-L1ADIpocytes,Daidzeininversestheattenuationofadiponectingeneexpressionbyco-culture,andtheseeffectsareinhibitedbythePPAR-γspecificinhibitor.Daidzeinattenuatesthereductionofadiponectinexpressioninadipocytes,andaPPAR-γspecificinhibitorabrogatedthiseffect.DirectactivationofPPAR-αand-γbyDaidzeinisconfirmedbyaluciferasereporterassay.InHEK293Tcells,DaidzeinsignificantlyincreasesPPAR-αtranscriptionalactivityinaconcentration-dependentmanner.Althoughanobviousdose-dependencyisnotobservedinPPAR-γtranscriptionalactivity,DaidzeinalsosignificantlyincreasesPPAR-γtranscriptionalactivityoverasimilarrangeofconcentrationsatwhichDaidzeinenhancedPPAR-αtranscriptionalactivity,withamaximumincreaseat25μM[1].Daidzeinisasoyisoflavone,whichupregulatestheexpressionofAbcg1,anditpromotesaxonaloutgrowthinculturedhippocampalneuronsviaestrogenreceptorsignaling.Daidzeinisamajorcomponentofsoywithstructuralsimilaritytoestrogen.Itexertsananti-inflammatoryeffect,lowerslipidlevels,andincreasesmitochondrialbiogenesis.Asanactivatorofnuclearreceptorperoxisomeproliferator-activatedreceptors(PPARs),DaidzeinenhancestranscriptionofPPARs-dependentgenes,includingliverXreceptors(LXRs,Nr1hgenefamilyinmice).IncubationwithdifferentconcentrationsofDaidzein,from5to100μM,increasesAPOEtranscriptionalactivity[2].

InVivo

TreatingApoeKOmicewithDaidzeinincreasesLxrandAbca1geneexpressionat1monthafterstroke,showingthattheabsenceofApoEdoesnotinterferewithothercholesterolhomeostasisgeneticprograms.Therefore,thefindingssuggestthatDaidzein-inducedApoEupregulationisacriticalcomponentinfosteringfunctionalrecoveryinchronicstroke[2].

ClinicalTrial
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References

  • [1].SakamotoY1,etal.TheDietaryIsoflavoneDaidzeinReducesExpressionofPro-InflammatoryGenesthroughPPARα/γandJNKPathwaysinAdipocyteandMacrophageCo-Cultures.PLoSOne.2016Feb22;11(2):e0149676.

    [2].KimE,etal.DaidzeinAugmentsCholesterolHomeostasisviaApoEtoPromoteFunctionalRecoveryinChronicStroke.JNeurosci.2015Nov11;35(45):15113-26.

PreparingStockSolutions
ConcentrationVolumeMass1mg5mg10mg
1mM3.9333mL19.6665mL39.3329mL
5mM0.7867mL3.9333mL7.8666mL
10mM0.3933mL1.9666mL3.9333mL
Pleaserefertothesolubilityinformationtoselecttheappropriatesolvent.
CellAssay
[1]

DaidzeinisdissolvedinDMSOandthendilutedwithappropriatemedia[1].

HEK293Tcellsareplatedon24-wellplatesatacelldensityofapproximately2.5×104cells/wellandaregrownto70-80%confluence.CellsarethentransientlytransfectedwithaPPAR-αorPPAR-γexpressionplasmid,andaplasmidcontainingtheluciferasegeneunderthecontrolofthreetandemPPARresponseelements(PPRE×3TK-luciferase)usinganX-tremeGENEHPDNATransfectionReagent.Renillaluciferasecontrolvectorsareco-transfectedtocontrolfortransfectionefficiency.Aftertransfection,cellsareculturedforanother24hinmediumcontainingDMSOorvariousconcentrations(6.25,12.5,25μM)ofDaidzein.Cellsarelysed,andluciferaseactivityismeasuredandexpressedasfoldinduction,thatisnormalizedtotheactivityoftherenillaluciferasecontrolplasmid[1].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.

AnimalAdmiNISTration
[2]

Mice[2]
Experimentsareperformedin10-to11-week-oldmaleC57(C57bl/6)andApoeKO(C57background)mice.Forlong-termstrokerecovery,micereceiveMoxifloxacin(100mg/kg)for3d.Theprophylacticantibiotictreatmentisshowntoeffectivelyreducemortalityinananimalmodelofstrokebyattenuatingperipheralinfection.Inaddition,salineissubcutaneouslyadministereddaily,andhydrogel(ClearH2O)isgiventopreventdehydration.Withtheimplementationofpoststrokecare(antibioticregimen,rehydration,andfeedinghydrogelswithsoftdiet)duringtheacuteperiod(<1 week),="" mice="" start="" to="" regain="" their="" body="" weight="" by="" day="" 5="" and="" continue="" to="" recover="" from="" stroke.="" animals="" are="" randomly="" selected="" for="" vehicle="" or="" daidzein="" treatment.="" vehicle="" or="" daidzein="" (10="" mg/kg)="" is="" administered="" subcutaneously="" within="" 30="" min="" of="" reperfusion="" after="" confirming="" the="" reperfusion="" of="" blood="" flow,="" daily="" for="" 7="" d="" and="" then="" every="" other="" day="" up="" to="" 1="" month.="" mce="" has="" not="" independently="" confirmed="" the="" accuracy="" of="" these="" methods.="" they="" are="" for="" reference="">

References

  • [1].SakamotoY1,etal.TheDietaryIsoflavoneDaidzeinReducesExpressionofPro-InflammatoryGenesthroughPPARα/γandJNKPathwaysinAdipocyteandMacrophageCo-Cultures.PLoSOne.2016Feb22;11(2):e0149676.

    [2].KimE,etal.DaidzeinAugmentsCholesterolHomeostasisviaApoEtoPromoteFunctionalRecoveryinChronicStroke.JNeurosci.2015Nov11;35(45):15113-26.

MolecularWeight

254.24

Formula

C₁₅H₁₀O₄

CASNo.

486-66-8

Storage
Powder-20°C3years
 4°C2years
Insolvent-80°C6months
 -20°C1month
Shipping

RoomtemperatureincontinentalUS;mayvaryelsewhere

Solvent&Solubility

10mMinDMSO

*"<1 mg/ml"="" means="" slightly="" soluble="" or="" insoluble.="" "≥"="" means="" soluble,="" but="" saturation="">

Purity:99.69%