Medchemexpress/Z-VAD-FMK(Synonyms: Z-VAD(OMe)-FMK; Z-Val-Ala-Asp(OMe)-FMK)/HY-16658/5mg-免疫在线蚂蚁淘旗下平台

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商品详细Medchemexpress/Z-VAD-FMK(Synonyms: Z-VAD(OMe)-FMK; Z-Val-Ala-Asp(OMe)-FMK)/HY-16658/5mg

Medchemexpress/Z-VAD-FMK(Synonyms: Z-VAD(OMe)-FMK; Z-Val-Ala-Asp(OMe)-FMK)/HY-16658/5mg

商品编号： HY-16658-10mM*1mLinDMSO

品牌： MedChemExp

市场价： ¥3700.00

美元价： 2220.00

产地：美国(厂家直采)

公司：

产品分类：小分子

公司分类： Small_molecule

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

立即询价

商品介绍

Z-VAD-FMKisacell-permeable,pan-caspaseinhibitor.

CustomerValidation

•CancerLett.2017Feb16;393:22-32.
•CancerLett.2016Aug28;379(1):134-142.
•AnalChem.2017Sep19;89(18):9788-9796.
•EnvironSciTechnol.2017Dec5;51(23):13938-13948.
•CellDeathDis.2017May25;8(5):e2810.
•BrJPharmacol.2017Sep;174(17):2941-2961.

•MolOncol.2017Aug;11(8):1035-1049.
•Oncotarget.2017May9;8(19):31297-31304.
•Oncotarget.2016Dec20;7(51):84810-84825.
•SciRep.2017Nov23;7(1):16111.
•SciRep.2017Oct19;7(1):13571.
•SciRep.2017Jun7;7(1):2929.
•SciRep.2016Dec1;6:38267.
•ToxicolApplPharmacol.2016Feb1;292:56-64.
•CancerMed.2017Feb;6(2):471-482.
•JEthnopharmacol.2017Aug12;212:8-17.
•BiomedPharmacother.2017Apr5;90:446-454.
•OncolRep.2017Feb;37(2):1270-1276.
•BiochemBiophysResCommun.2016Mar18;471(4):539-44.
•ChineseJournalofCellBIOLOGy.2016,38(10):1232-1243.

Description	Z-VAD-FMKisacell-permeable,pan-caspaseinhibitor.
IC₅₀&Target	pan-caspase^[1]
InVitro	Z-VAD-FMKisabroad-spectrumcaspaseinhibitor,hasbeenshowntoinhibittheintracellularactivationofcaspase-likeproteases.TheinjectionofZ-VAD-FMKsuppressesthecaspase-3activityinlungtissues,andsignificantlydecreasesthenumberofterminaldUTPnick-endlabeling-positivecells^[1].Z-VAD-FMKisadmiNISTeredintraperitoneallyat1hourbeforeand6hoursafterSAH.Expressionofcaspase-3andpositiveTUNELisexaminedasMarkersforapoptosis.Z-VAD-FMKsuppressesTUNELandcaspase-3staininginendothelialcells,decreasescaspase-3activation,reducesBBBpermeABIlity,relievesvasospasm,abolishesbrainedema,andimprovesneurologicaloutcome^[2].Z-VAD-FMKisacell-permeablecaspaseinhibitor,efficientlyblockscelldeathinducedbySMNdeficiency^[3].
InVivo	ThesurvivalrateofmiceisprolongedsignificantlybytheinjectionofZ-VAD-FMK.AllmicesuccumbedtoLPSwithin30hours.Bycontrast,themicetreatedwithZ-VAD-FMKsurvivesignificantlylongerand27%ofthemicesurvivedmorethan7days^[1].
References	[1].KawasakiM,etal.Protectionfromlethalapoptosisinlipopolysaccharide-inducedacutelunginjuryinmicebyacaspaseinhibitor.AmJPathol.2000Aug;157(2):597-603. [2].ParkS,etal.Neurovascularprotectionreducesearlybraininjuryaftersubarachnoidhemorrhage.Stroke.2004Oct;35(10):2412-7. [3].IlangovanR,etal.InhibitionofapoptosisbyZ-VAD-fmkinSMN-depletedS2cells.JBiolChem.2003Aug15;278(33):30993-9.

PreparingStockSolutions

ConcentrationVolumeMass	1mg	5mg	10mg

1mM	2.1391mL	10.6954mL	21.3908mL
5mM	0.4278mL	2.1391mL	4.2782mL
10mM	0.2139mL	1.0695mL	2.1391mL

Pleaserefertothesolubilityinformationtoselecttheappropriatesolvent.

CellAssay
^[3]

Z-VAD-FMKisdissolvedinDMSOandstored,andthendilutedwithappropriatemediumbeforeuse^[3].

PCRproductscontainingcodingsequencesforthedSMN(forwardprimer:5′-TAATACGACTCACTATAGGGAAGACGTACGACGAGTCG-3′;andreverseprimer:5′-TAATACGACTCACTATAGGGGTGGTGCTGGCTTCTTTC-3′;productlength,601bps;boldanditalicslettersrepresentT7promotersequences)andcontrolDrosophilaPresenilin(dPsn)gene(forwardprimer:5′-TAATACGACTCACTATAGGGTGGCTGCTGTCAATCTC-3′;andreverseprimer:5′-TAATACGACTCACTATAGGGCGATAGCAACGCTTCTTG-3′;productlength:543bps)areobtainedandgel-purified.Double-strandedRNAs(dsRNA)aregeneratedbytranscriptionwithRibomaxT7TranscriptionkitanddigestedwithRnase-freeDNase.ThedsRNAproductsareethanolprecipitatedandannealedbyincubationat65°Cfor30minandthenslowlyallowedtocoolatroomtemperature.TheannealeddsRNAproductsareanalyzedona1%agaorsegeltoensurethemajorityofdsRNAexistedasasingleband.ThedsRNA(2μg)and/orplasmidDNAs(2μg)areintroducedintocellsbyusingCellfectin.Caspaseinhibitionisachievedbyusing50μMofZ-VAD-FMKintheculturemedium^[3].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.

AnimalAdministration
^[1]^[2]

Z-VAD-FMKisdissolvedat2mg/mLin1%DMSOinsterilesaline(Mice)^[1].
Z-VAD-FMKisdissolvedin1%DMSOandfurtherdilutedinphysiologicalbuffersolution(final<0.01% dmso)="">^[2].

Mice^[1]
Miceusedinthisstudyare5-to6-week-old(20to22g)ICRmales.Miceareinjectedwith30mg/kgLPSfromE.coliSEROtypeO111:B4throughthetailvein.AsingleintravenousinjectionofZ-VAD.fmk(0.25mg)ismade15minutesbeforeLPSinjection,followedbythreeintravenousinjectionsofZ-VAD-FMK(0.1mgeach)perhour.Controlmiceareinjectedwiththesamevolumeof1%DMSOinsterilesaline.
Rat^[2]
MaleSprague-Dawleyratsweighing300to350gareanesthetizedwithα-chloralose(40mg/kgIP)andurethane(400mg/kgIP).Animalsareintubated,andrespirationismaintainedwithasmallanimalrespirator.Rectaltemperatureismaintainedat37±0.5°Cwithaheatingpad.Theleftexternalcarotidarteryisisolatedanda4.0monofilamentnylonsutureisinsertedthroughtheinternalcarotidarterytoperforatethemiddlecerebralartery.SAHisconfirmedatautopsyineachrat.Sham-operatedratsunderwentthesameproceduresexceptthatthesutureiswithdrawnafterresistanceisfelt.Z-VAD-FMK(50μMper0.3mL)isinjectedintraperitoneallyat1hourbeforeand6hoursafterSAHinduction.Invehiclegroup,ratsunderwentSAHinductionandaretreatedwiththesamevolumeofvehicle(DMSOdilutedinphysiologicalbuffersolution).Notreatmentisappliedinsham-operatedanimals.MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.

References

[1].KawasakiM,etal.Protectionfromlethalapoptosisinlipopolysaccharide-inducedacutelunginjuryinmicebyacaspaseinhibitor.AmJPathol.2000Aug;157(2):597-603.
[2].ParkS,etal.Neurovascularprotectionreducesearlybraininjuryaftersubarachnoidhemorrhage.Stroke.2004Oct;35(10):2412-7.
[3].IlangovanR,etal.InhibitionofapoptosisbyZ-VAD-fmkinSMN-depletedS2cells.JBiolChem.2003Aug15;278(33):30993-9.

MolecularWeight

467.49

Formula

C₂₂H₃₀FN₃O₇

CASNo.

187389-52-2

Storage

Powder	-80°C	2years
	-20°C	1year
Insolvent	-80°C	6months
	-20°C	1month

Shipping

RoomtemperatureincontinentalUS;mayvaryelsewhere

Solvent&Solubility

DMSO:≥30mg/mL

*"<1 mg/ml"="" means="" slightly="" soluble="" or="" insoluble.="" "≥"="" means="" soluble,="" but="" saturation="">

Purity:>98.0%

SelectBatch: